Magnitude of CD8 cell reactivity with a peptide pool is comparable to the sum of responses by individual peptides

نویسندگان

  • Tameem Ansari
  • Wenji Zhang
  • Ioana Moldovan
  • OLeg Targoni
  • Ramu A Subbramanian
  • Paul V Lehmann
چکیده

Methods Using a model peptide pool that is comprised of 32 welldefined viral epitopes from Cytomegalovirus, Epstein-Barr virus, and Influenza virus (CEF peptide pool), we assessed peptide competition in PBMC from 43 human subjects. IFN-g was measured using the ImmunoSpot Test Kit, and to assure low background, serum-free, CTL-TestTM Medium was used. The spots were counted using an ImmunoSpot S6 Core reader.

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عنوان ژورنال:

دوره 1  شماره 

صفحات  -

تاریخ انتشار 2013